chronicle of a soil bacterium: paenibacillus polymyxa e681 as a recent paradigm shift upon revisiting the role of soil microbes in plant health suggests that the rhizosphere microbiome is a key determinant of plant health (berendsen et al., 2012). plant growthpromoting rhizobacteria (pgpr), which colonize plant roots, draw special attention due to their beneficial effects on crops and ecosystems (kloepper et al., 2004). rhizosphere bacteria strains of various genera are designated pgpr. the fluorescent pseudomonads have received special interest due to their versatile metabolism, rapid growth, and strong mobility (bakker et al., 2007). streptomycetaceae are exceptional antibiotic producers and can suppress plant pathogens. however, the major barriers to agricultural usage of streptomycetes spp. and pseudomonas spp. are difficulties associated with mass production in liquid culture, and poor longterm storage due to a short shelflife, respectively (emmert and handelsman, 1999). bacillus spp. and paenibacillus spp. are strong candidates for ov see full list on frontiersin as mentioned above, p. polymyxa strains have been mostly isolated from soil and rhizosphere, the presumed natural habitats of p. polymyxa. for instance, the model strain p. polymyxa e681 was isolated from roots of winter barley in south korea (ryu and park, 1997ryu et al., 2005b). among 3179 endosporeforming bacteria isolated after heat treatment at 80°c for 1 h, 31 isolates antagonistic against oomycetes pathogens, particularly against pythium spp. and phytophthora spp. were obtained. strain e681 was eventually selected based on its aggressive root colonization capacity compared with other bacillales species (choi et al., 2004), and its strong antagonism against a broad spectrum of fungi, oomycetes, and bacteria in vitro. as shown in figure 1, our previous studies demonstrate that strain e681 promotes plant growth when seeds are soaked in bacterial suspension, displays antagonism against plant pathogenic fungi, oomycetes, and bacteria, produces various extracellular hydrolytic e see full list on frontiersin in this section, we summarize evidence showing that p. polymyxa e681 promotes plant growth and protects against plant pathogens, and compare its properties with those of other strains of p. polymyxa. the possible mechanisms underpinning plant responses to p. polymyxaare also discussed. see full list on frontiersin genome sequencing of p. polymyxa e681 in the 1990s, our group participated as a member of the international consortium in genome sequencing of the grampositive model organism bacillus subtilis (kunst et al., 1997) using classical clonebased sanger sequencing methodologies. despite a positive outcome and compatibility with genomic research on the genus bacillus and related microbes, obtaining complete bacterial genomes remained challenging until the advent of nextgeneration sequencing technologies that became available in the e requirement for a new phylogenomic position for strain e681 and other related paenibacillus spp. strains early taxonomic allocation of e681 to p. polymyxa species was solely based on 16s rrna gene sequence analysis. however, recent species demarcation approaches allow strains sharing genome similarity with the type strain of a particular species above a certain criterion to be assigned that species name. genome similarity is often expressed as ani or other measures (goris et al., 2007richter and rossellomora, 2009), and 95 or 96% pairwise ani values are commonly adopted as thresh values. s see full list on frontiersin genome sequencing of p. polymyxa strains revealed numerous antibiotic biosynthetic genes in the genome encoding nrpss, polyketide synthases (pkss), and bacteriocins. nrps and pks generally have a modular structure, with the modules containing multiple domains responsible for recognition, activation and condensation of an amino acid and an acylcoa (aleti et al., 2015). an nrps module typically includes at least one condensation (c) domain, an adenylation (a) domain, and a thiolation (t) domain. other domains including those for epimerization (e) and termination (te) domains also contribute to antibiotic synthesis. figure 3 shows bgcs for antimicrobials characterized in strain e681. these diverse antibiotic production machineries presumably help to fight against competitors that are harmful to plants in the environment, making them useful for plant protection. in addition, some antibiotics have been used for medical purposes for the treatment of mdr bacteria. according to the antisma see full list on frontiersin regulation of sporulation an indepth understanding on the regulation of p. polymyxa sporulation is critical for developing industrial applications based on this strain, especially as continuous cultivation prohibits sporulation during the fermentation process. sporulation initiation in bacillus and related organisms including paenibacillus spp. is dependent on a wellconserved multicomponent phosphorelay system. multiple histidine kinases sense environmental sporulation signals and autophosphorylate themselves. the p active inclusion body formation overexpression of recombinant proteins in e. coli often results in inclusion bodies in which misfed proteins are aggregated and biologically inactive. this is commercially disadvantageous because solubilization of the inclusion bodies and refing of the proteins are costly. therefore, the formation of biologically active inclusion bodies in e. coli is attractive from a commercial perspective. p. polymyxa pyruvate oxidase (poxb) forms large inclusion bodies when overexpressed in e. coli ( boosting the strength of cement paste one interesting study demonstrated the industrial applicability of strain e681 (park et al., 2014). cement paste containing strain e681 displayed strong antifungal activity against aspergillus niger, a deleterious fungus commonly found in cement buildings and structures. strain e681 was also shown to help repair cracks by precipitation of calcium carbonate crystals in cement paste. these results suggest that strain e681 could be used in the development of multifunctional cement mortar. see full list on frontiersin herein, we summarized literature demonstrating that information derived from previous studies on p. polymyxae681 provide an important gateway for promoting plant health and human healthcare. strain e681 was initially studied as an outstanding pgpr based on its potent plant growthpromoting and plantprotecting activities, and bioinformatic and functional analyses of its genome subsequently illuminated various characteristics that benefit plants. more recently, strain e681 was found to be adept at producing various antibiotics, most notably polymyxins, recently reinstated as a therapy of last resort for treating gramnegative infections. other antibiotics produced by e681 include tridecaptin, paenilan, paenilipoheptin, and a putative polyketide, all of which are worthy of further study for the development of novel antibiotics against mdr human pathogenic bacteria. fusaricidins elicit strong isr effects, making them attractive as plant protectant agents in agriculture. a recombinant see full list on frontiersin hj conceived the project. hj and shp sequenced the genomes and conducted comparative analysis. all authors wrote the manuscript and reviewed the final version of the manuscript prior to submission for publication. see full list on frontiersin this research was supported by the korea research institute of bioscience and biotechnology (kribb) initiative program, the bio medical technology development program of the national research foundation (nrf) funded by the korean government (msit) (nrf2018m3a9f3079565), and the strategic initiative for microbiomes in agriculture and food, ministry of agriculture, food and rural affairs, south korea [as part of the (multiministerial) genome technology to business translation program 9180174], south korea. see full list on frontiersin the authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. see full list on frontiersin mechanisms of adhesion of paenibacillus polymyxa onto mechanisms of adhesion of paenibacillus polymyxa onto hematite, corundum and quartz narnita deo a, k.a. natarajan b, p. somasundaran a.. received 15 august 1999~cepted 17 july 2000 abstract adhesion of bacteria onto solid surfaces is a necessary event in nature for the utilization of namita deo columbia university, ny cu langmuir center fundamental questions arise on the mechanisms of complexation of the polyelectrolyte and surfactant that control their behavior. mechanisms of adhesion of paenibacillus polymyxa onto hematite biowzbogacanie surowców mineralnych interakcja bakteria mechanisms of adhesion of paenibacillus polymyxa onto hematite, corundum and quartz. int. j. miner. process., 62, 2739 reversibility and mechanism of bacterial international journal of mineral processing minerals read the latest articles of international journal of mineral processing at sciencedirect , elseviers leading platform of peerreviewed scholarly literature 3mechanisms of adhesion of paenibacillus polymyxa onto mechanisms of adhesion of paenibacillus polymyxa onto hematite, corundum and quartz deo, namita natarajan, k. a. somasundaran, p. (2001) mechanisms of adhesion of paenibacillus polymyxa onto hematite, corundum and quartz international journal of mineral processing, 62 (14). pp. 2739. draft genome sequences of pantoea agglomerans, paenibacillus we report here the draft genome sequences of strains of pantoea agglomerans (ekm10t, ekm20t, ekm21t, and ekm22t), paenibacillus polymyxa (ekm10p and ekm11p), and pseudomonas sp. strain ekm23d. these microbes were cultured from fresh seed biogels of cucumis sativus l. (cucumber) and cucumis melo l. (cantaloupe). the strains suppress the growth of soilborne fungal/oomycete phytopathogens in vitro .
adhesion of paenibacillus polymyxa on chalcopyrite and pyrite the adhesion behaviour of paenibacillus polymyxa bacteria on pyrite and chalcopyrite is examined by the surface thermodynamics and the extended dlvo theory approaches. in addition, the bacteria are mechanisms of adhesion of paenibacillus polymyxa onto request pdf mechanisms of adhesion of paenibacillus polymyxa onto hematite, corundum and quartz adhesion of bacteria onto solid surfaces is a necessary event in nature for the utilization of fundamental aspects of hematite flotation sciencedirectmay 01, 2015 · the effect of p. polymyxa onto hematite was studied by deo et al. (2001), and they showed that the flotation recovery of hematite was unaffected by increasing the cell number. at a cell density of 1.5 × 10 9 cells/ml, only 9% of the hematite sample was recovered in the float fraction. bioinspired adhesive and antibacterial microneedles for here, inspired by the antibacterial strategy of paenibacillus polymyxa and adhesion mechanisms of mussel byssi and octopus tentacles, we develop hierarchical microneedles with multifunctional adhesive and antibacterial abilities. with polydopamine hydrogel as the microneedle base and a loop of suctioncupstructured concave chambers encircling the ph dependence of escherichia coli o157:h7 adsorption on jul 31, 2014 · deo n, natarajan k, somasundaran p (2001) mechanisms of adhesion of paenibacillus polymyxa onto hematite, corundum and quartz. int j miner process 62:2739 int j miner process 62:2739 cas article google scholar mechanisms of adhesion of paenibacillus polymyxa onto coremechanisms of adhesion of paenibacillus polymyxa onto hematite, corundum and quartz adhesion of bacteria onto solid surfaces is a necessary event in nature for role of biopolymers on bacterial adhesion and mineral interactions between paenibacillus polymyxa, with different minerals such as hematite, corundum and quartz are examined in this work in the light of derjaguin, landau, verwey and overbeek theory
interactional mechanisms of paenibacillus polymyxa sc2 and paenibacillus polymyxa sc2, a bacterium isolated from the rhizosphere soil of pepper (capsicum annuum l.), promotes growth and biocontrol of pepper. however, the mechanisms of interaction between p. polymyxa sc2 and pepper have not yet been elucidated. mechanisms of adhesion of paenibacillus polymyxa onto mechanisms of adhesion of paenibacillus polymyxa onto hematite, corundum and quartz adhesion of bacteria onto solid surfaces is a necessary event in nature for namita deo, ka natarajan and p somasundaranmechanisms of adhesion of paenibacillus polymyxa onto may 01, 2001 · interactions between paenibacillus polymyxa, with different minerals such as hematite, corundum and quartz are examined in this work in the light of derjaguin, landau, verwey and overbeek theory, popularly known as dlvo theory and possible chemical interactions. the adhesion process is normally controlled initially by physicochemical namita deo, k.a natarajan, p somasundaran 90 2001exopolysaccharides of paenibacillus polymyxa rhizobacteria in may 22, 2013 · deo n, natarajan ka, somasundaran p (2001) mechanisms of adhesion of paenibacillus polymyxa onto hematite, corundum and quartz. int j miner process 62:2739 crossref google scholar dickschat js (2010) quorum sensing and bacterial biofilms. (pdf) the role of interaction between paenibacillus polymyxa a successful adsorption of the paenibacillus  el geassy a., nasr m. i., yousef m. a., khedr m. h., and polymyxa bacterial strain onto (hematite bahgat m., 2008, "behavior of manganese oxides pyrolusite) surfaces caused a degree of during magnetizing reduction of bahariya iron ore by aggregation for minerals particles leading to a co co2 adhesion of paenibacillus polymyxa on chalcopyrite and pyrite may 15, 2003 · the adhesion behaviour of paenibacillus polymyxa bacteria on pyrite and chalcopyrite is examined by the surface thermodynamics and the extended dlvo theory approaches. in addition, the bacteria are adapted to pyrite and chalcopyrite minerals, and the adhesion behaviour of these bacteria is also investigated. p.k. sharma, k. hanumantha rao 231 2003